Thermo Fisher Scientific CD8 Monoclonal Antibody (C8/144B)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100–1:500
• Application Note: Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Flow Cytometry (Flow)

• Tested Dilution: 2.5 µL/1x10^6 cells

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt.
• For IHC, Tris-EDTA pH 9.0 epitope retrieval is recommended for FFPE tissue sections.

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin family that plays a key role in T cell signal transduction, differentiation, and activation.
CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer (CD8α/CD8β), functioning as a co-receptor with the T cell receptor (TCR) for MHC class I molecules.
It is also expressed on subsets of T cells, NK cells, monocytes, and dendritic cells as CD8α homodimers.
Engagement of MHC-I/peptide complexes by CD8 triggers Lck recruitment, leading to lymphokine production, motility, and cytotoxic T lymphocyte (CTL) activation.
Activated CTLs are crucial for pathogen and tumor cell clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.