
Thermo Fisher Scientific CD8 Monoclonal Antibody (C8/144B)
CD8 단백질을 인식하는 마우스 모노클론 항체로 인간 시료에 반응. IHC 및 유세포분석(Flow Cytometry)에 적합. 비접합(unconjugated) 형태로 제공되며, 고순도 Protein G 정제. 연구용으로만 사용 가능.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:100–1:500
- Application Note: Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Flow Cytometry (Flow)
- Tested Dilution: 2.5 µL/1x10^6 cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | C8/144B |
| Immunogen | 13 amino acid synthetic peptide from the C-terminal cytoplasmic domain of alpha chain of human CD8 molecule |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Protein G |
| Storage Buffer | BBS, pH 8.2, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt.
- For IHC, Tris-EDTA pH 9.0 epitope retrieval is recommended for FFPE tissue sections.
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin family that plays a key role in T cell signal transduction, differentiation, and activation.
CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer (CD8α/CD8β), functioning as a co-receptor with the T cell receptor (TCR) for MHC class I molecules.
It is also expressed on subsets of T cells, NK cells, monocytes, and dendritic cells as CD8α homodimers.
Engagement of MHC-I/peptide complexes by CD8 triggers Lck recruitment, leading to lymphokine production, motility, and cytotoxic T lymphocyte (CTL) activation.
Activated CTLs are crucial for pathogen and tumor cell clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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