Invitrogen™

PureLink™ HQ Mini Plasmid DNA Purification Kit

The PureLink HQ Mini Plasmid Purification Kit is designedfor the isolation of high-quality plasmid DNA that is suitablefor restriction enzyme자세히 알아보기

The PureLink HQ Mini Plasmid Purification Kit is designedfor the isolation of high-quality plasmid DNA that is suitablefor restriction enzyme digestion, PCR, sequencing, bacterialcell transformation, and mammalian cell transfection.Using the kit, plasmid DNA can be isolated from varyingamounts of bacterial cells.

The PureLink HQ Mini Plasmid Purification Kit has thefollowing advantages:
• Higher spin column capacity for plasmid DNA ascompared to other commercially available plasmidpurification systems
• Designed to isolate high-quality plasmid DNA in less thanan hour
• Minimal genomic DNA contamination of the purifiedsample
• Reliable performance of the purified DNA in downstreamapplications

Process overview
Bacterial cells are pelleted, resuspended, and lysed. Thelysate is then neutralized and conditions are adjusted forsubsequent binding. After clarification by centrifugation, thelysate is processed through the PureLink spin column. TheDNA binds to the silica-based membrane in the column, andimpurities are removed by a single wash step. The DNA isthen eluted in Elution Buffer or water.

사양

컬럼 유형Spin Column

형식Spin Column

Isolation TechnologySilica Spin Column

스케일Mini

샘플 종류Bacterial Culture

최종 제품 유형Plasmid DNA

용도(애플리케이션)PCR, Transfection, Sequencing, Transformation, Cloning

고처리량 호환성Not High-throughput Compatible (Manual)

반응 수100 Preps

프렙 스케일< 100 μg (Small-Scale) Plasmid DNA

제품라인PureLink™

제품 유형Plasmid DNA Purification Kit

수량100 Preps

배송 조건Room Temperature

타겟Plasmid DNA

Unit SizeEach