Invitrogen™

TA Cloning™ Kit, Dual Promoter, with pCR™II Vector and One Shot™ INVαF` Chemically Competent E. coli

The TA Cloning™ Kit Dual Promoter with pCR™II vector provides a quick, one-step cloning strategy for directly inserting a Taq-amplified자세히 알아보기

The TA Cloning™ Kit Dual Promoter with pCR™II vector provides a quick, one-step cloning strategy for directly inserting a Taq_-amplified PCR product into a plasmid vector. The T7 and Sp6 promoters of the pCR™II vector allow _in vitro transcription of the insert to produce sense or anti-sense products. The TA Cloning Kit Dual Promoter uses the pCR™II cloning vector and ExpressLink™ T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.

Features of the TA Cloning™ Kit Dual Promoter:
• Fast & convenient—15-minute, room-temperature ligation
• Efficient—blue/white screening and >80% clones with correct insert
• Flexible—choice of kanamycin or ampicillin resistance for flexible antibiotic selection
• Hassle-free—eliminates any enzymatic modifications of the PCR product
• Streamlined—does not require the use of PCR primers that contain restriction sites

The pCR™II vector provides:
• 3`-T overhangs for direct ligation of Taq_-amplified PCR products
• T7 and Sp6 promoters for _in vitro RNA transcription and sequencing
• Versatile polylinker with flanking _Eco_R I sites for easy excision of inserts
• M13 forward and reverse primer sites for sequencing

How TA Cloning™ Works
Taq polymerase has a non-template-dependent activity that adds a single deoxyadenosine (A) to the 3ends of PCR products. The linearized vector supplied in this kit has single 3 deoxythymidine (T) residues. This allows PCR inserts to ligate efficiently with the vector.

Kit Configurations
The TA Cloning™ Kit is offered in a variety of configurations: with One Shot™ INVFChemically Competent _E. coli_ (K2050-01 and K2050-40), One Shot™ TOP10F Chemically Competent E. coli (K2060-01 and K2060-40), and without competent cells (K2750-20 and K2750-40) in 20- and 40-reaction kit sizes.

사양

박테리아 또는 효모 균주INVαF ́

세포 유형Chemically Competent E. coli

클로닝 방법TA Cloning

형식Kit

용도(애플리케이션)PCR Cloning

포함Linearized pCRII vector, ExpressLink T4 DNA ligase, 5X ExpressLink T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, controls, One Shot INVαF` chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

반응 수20 Reactions

제품라인One Shot™

제품 유형Cloning Kit

프로모터T7, SP6

수량20 reactions

벡터pCRII

Unit SizeEach