Thermo Fisher Scientific Vinculin Monoclonal Antibody (C4)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1 µg/mL

-

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Guinea pig, Human, Mouse, Pig, Rabbit, Rat

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

C4

Immunogen

Recombinant Human/Mouse/Rat VCL protein, Lys1020-Gln1134 (Accession #P18206) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Vinculin,uniProtId:P18206-1,ncbiNodeId:9606,antigenRange:1020-1134,antigenLength:1134,antigenImageFileName:MA5-50897_Vinculin_P18206-1_House_mouse.svg,antigenImageFileNamePDP:MA5-50897_Vinculin_P18206-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.05% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3094394

Target Information

Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.