Invitrogen™

pBAD/gIII Kit

The pBAD/gIII Kit provides all of the necessary reagents to express recombinant proteins under the tight regulation of the araBAD자세히 알아보기

The pBAD/gIII Kit provides all of the necessary reagents to express recombinant proteins under the tight regulation of the araBAD promoter. To simplify purification, the vector pBAD/gIII encodes a leader peptide which directs the recombinant protein into the
periplasmic space. The vector provides:

• The araBAD promoter for tightly regulated expression
• Translation initiation signals for optimized E. coli_expression
• Leader peptide from the bacteriophage fd gene III protein (gIII) for secreted expression
• C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin
• C-terminal _c-myc epitope for detection and analysis with an Anti-myc Antibody

A set of three vectors is provided (A, B, and C). Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of your gene.

사양

구성 또는 유도성 시스템Inducible

유도제Arabinose

프로모터araBAD

제품 유형Bacterial Expression Vector

선택 제제(진핵)None

항생제 내성 박테리아Ampicillin (AmpR)

단백질 태그c-Myc Epitope Tag

클로닝 방법Restriction Enzyme/MCS

수량1 kit

벡터pBAD

Unit Size1 kit