Invitrogen™

pBAD/His Kit

The pBAD/His Kit provides all of the necessary reagents to express your protein in a tightly regulated fashion. The vector자세히 알아보기

The pBAD/His Kit provides all of the necessary reagents to express your protein in a tightly regulated fashion. The vector pBAD/His allows you to express your protein with an N-terminal tag. The vector provides:

• The araBAD promoter for tightly regulated expression
• Translation initiation signals optimized for _E. coli_expression
• N-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin and detection with an Anti-HisG Antibody
• N-terminal Xpress™ epitope for detection and analysis with an Anti-Xpress™ Antibody
• Enterokinase cleavage site for removing the N-terminal tag following purification

Three vectors are provided (A, B, and C). Each has the N-terminal tag in a different reading
frame relative to the multiple cloning site to simplify in-frame cloning of your gene.

사양

구성 또는 유도성 시스템Inducible

유도제Arabinose

프로모터araBAD

제품 유형Bacterial Expression Vector

선택 제제(진핵)None

항생제 내성 박테리아Ampicillin (AmpR)

분열EK (Enterokinase) Recognition Site

단백질 태그His Tag (6x)

클로닝 방법Restriction Enzyme/MCS

수량1 kit

벡터pBAD

Unit Size1 kit