Invitrogen™

pBAD/_Myc_-His Kit

The pBAD/Myc-His Kit provides all of the necessary reagents to express your protein in a tightly regulated fashion. The pBAD/Myc-His자세히 알아보기

The pBAD/_Myc_-His Kit provides all of the necessary reagents to express your protein in a tightly regulated fashion. The pBAD/_Myc_-His vector expresses native proteins or fusion proteins with a C-terminal tag. The vector provides:

• The araBAD promoter for tightly regulated expression
• Translation initiation signals optimized for E. coli_expression
• C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin or detection with an Anti-His(C-term) Antibody
• C-terminal _c-myc epitope for detection and analysis with an Anti-myc Antibody

Three vectors are provided (A, B, and C). Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of your gene.

사양

구성 또는 유도성 시스템Inducible

유도제Arabinose

프로모터araBAD

제품 유형Bacterial Expression Vector

선택 제제(진핵)None

항생제 내성 박테리아Ampicillin (AmpR)

분열EK (Enterokinase) Recognition Site

단백질 태그c-Myc Epitope Tag

클로닝 방법Restriction Enzyme/MCS

수량1 kit

벡터pBAD

Unit Size1 kit