Thermo Fisher Scientific KSR1 Polyclonal Antibody
KSR1 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, WB, ICC/IF, ELISA에 사용 가능. 인간, 마우스, 랫트 반응성. 액상 형태로 -20°C 보관. 세포 내 MAPK 경로 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 320–420 of human KSR1 (NP_055053.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.69 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913903 |
Product Specific Information
Positive test controls include:
- U-251MG
- HeLa
- Jurkat
- Mouse brain, spleen, testis, kidney, lung
- Rat brain
Localization: Cytoplasm, Endoplasmic reticulum membrane, Membrane, Peripheral membrane protein.
Immunogen sequence:
SSPAPFPTSS NPSSATTPPN PSPGQRDSRF NFPAAYFIHH RQQFIFPVPS AGHCWKCLLI AESLKENAFN ISAFAHAAPL PEAADGTRLD DQPKADVLEA H
Target Information
Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases. Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2. Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP. Its kinase activity is uncertain; some protein kinase activity has been detected in vitro, however the physiological relevance of this activity is unknown. [UniProt]
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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