Thermo Fisher Scientific CD47 Recombinant Rabbit Monoclonal Antibody (006)

Applications

• ELISA (ELISA)
  Tested Dilution: 1:5,000–1:10,000

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide (final concentration 0.05%–0.1%) to prevent contamination.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Improved specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broad immunoreactivity due to large rabbit immune repertoire

This antibody specifically recognizes Human CD47.

Target Information

CD47 (Integrin-Associated Protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein that interacts with several integrins and regulates their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, including activation of heterotrimeric G proteins.

Functionally, CD47 participates in increasing intracellular calcium concentration during cell adhesion to the extracellular matrix. It also acts as a receptor for the C-terminal cell-binding domain of thrombospondin, playing roles in membrane transport and signal transduction.

CD47 is widely distributed across tissues and shows reduced expression on Rh erythrocytes. It serves as a ligand for CD172a (SIRPα), an immune inhibitory receptor on macrophages, preventing phagocytosis of CD47-positive cells. The CD47–CD172a interaction influences cell migration, B cell adhesion, T cell activation, chondrocyte mechanotransduction, and neuronal development, being abundant in synapse-rich regions of the brain and retina.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.