Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Super Bright 780, eBioscience
마우스 CD45 전 아이소폼을 인식하는 30-F11 단클론 항체로, Super Bright 780 형광체로 결합되어 유세포분석에 적합합니다. 405 nm 레이저로 여기되며 780 nm에서 방출됩니다. 4°C 암소 보관, 동결 금지. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 78-0451-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Super Bright 780, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 16 publications |
| Immunohistochemistry (PFA fixed) | - | View 1 publication |
| Immunohistochemistry (Frozen) | - | View 3 publications |
| Immunocytochemistry (ICC/IF) | - | View 5 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 121 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Mouse |
| Host/Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 780, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 30-F11 |
| Conjugate | Super Bright™ 780 |
| Excitation/Emission Max | 413/780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2734987 |
Available Formats
Unconjugated, Alexa Fluor 488, 532, 561, 660, 700, APC, APC-eFluor 780, Biotin, Brilliant UV/Violet series, eFluor series, FITC, PE, PE-Cyanine series, PerCP-Cyanine5.5, Super Bright 436/600/645/702, NovaFluor series 등 다양한 형광체 포맷 제공.
Product Specific Information
The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except mature erythrocytes and platelets. It possesses tyrosine phosphatase activity and plays a key role in lymphocyte activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Validated for mouse bone marrow cells at ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
- Excitation/Emission: 405 nm / 780 nm (Violet laser)
- Recommended Filter: 780/60 bandpass
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific interactions.
For compensation, use cells rather than beads when possible (e.g., UltraComp eBeads or AbC Total Antibody Compensation beads).
Light Sensitivity: Protect from light due to photo-induced oxidation.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333).
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD45 (Leukocyte Common Antigen, LCA) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells. It regulates Src-family kinases, antigen receptor signaling, and cytokine receptor signaling. CD45 isoforms arise from alternative splicing and vary in extracellular domains. It is crucial for lymphocyte development, survival, and immune regulation through modulation of integrin-mediated pathways and apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.