Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), PE-Cyanine7, eBioscience
CD45.2 단백질을 인식하는 마우스 단클론 항체로, PE-Cyanine7 형광체가 결합되어 있습니다. Flow Cytometry용으로 검증되었으며, 주요 마우스 균주(C57BL/6 등)의 백혈구 표면 CD45.2 검출에 적합합니다. 고순도 정제 및 광민감성 주의 필요.
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- 25-0454-8x (2개 옵션)
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), PE-Cyanine7, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.5 µg/test | View 46 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 104 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573349 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 605 / 650 / 711 / 786
- eFluor 450 / 506
- FITC
- NovaFluor Blue 510 / 555 / 585 / 610-70S / 660-120S
- PE
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645 / 780
- Request custom conjugation
Product Specific Information
Description:
The 104 monoclonal antibody reacts with mouse CD45 molecule (leukocyte common antigen, LCA) in CD45.2-expressing mouse strains such as C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A, and AKR. CD45.2 is expressed by all leukocytes in these strains.
Applications Reported:
Validated for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.5 µg per test (100 µL final volume). Cell number can range from 10⁵–10⁸ cells/test. Optimal concentration should be titrated for specific assays.
Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-822-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and compensation efficiency.
Excitation / Emission:
488–561 nm / 775 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising about 10% of surface proteins in lymphocytes. It regulates Src-family kinases, influences lymphocyte development and antigen signaling, and contributes to integrin-mediated signal transduction and apoptosis regulation. CD45RA is an isoform with restricted expression among lymphoid subtypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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