Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), Brilliant Ultra Violet 737, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.5 µg/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496
• Brilliant UV 661
• Brilliant Violet 421
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The H1.2F3 monoclonal antibody reacts with mouse CD69 (very early activation antigen, VEA), a ~35 kDa disulfide-linked dimer expressed on a subset of lymphocytes in the thymus, spleen, and lymph nodes. Activation of T and B cells rapidly upregulates CD69 expression, indicating its role in lymphocyte development and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
  Optimal titration is recommended for best performance.

Fluorochrome Information:
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is intended for use with UV (355 nm) lasers. Verify instrument compatibility before use.

Buffer Recommendation:
For panels using multiple Brilliant Ultra Violet, Brilliant Violet, or polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Light Sensitivity:
Protect from light; the dye is sensitive to photo-induced oxidation.

Fixation:
Samples may be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer ([Product # 00-8222-49])
• 1-step Fix/Lyse Solution ([Product # 00-5333-54])

BUV737 is compatible with short-term methanol fixation but should not be stored in methanol-containing buffers for more than 1 hour.

Excitation: 355 nm
Emission: 732 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It contains N-linked oligosaccharides and is expressed on activated platelets. In peripheral blood, CD69 expression varies and is involved in lymphocyte signal transduction and proliferation. It may also mediate signaling in NK cells and platelets. Alternative splicing produces multiple transcript variants.

Associated Diseases: Coccidiodomycosis, Asthma

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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