Invitrogen™

Dynabeads™ Mouse Pan T (Thy1.2)

Dynabeads™ Mouse pan T (Thy 1.2) are magnetic beads that provide a robust and versatile tool to isolate or deplete자세히 알아보기

Dynabeads™ Mouse pan T (Thy 1.2) are magnetic beads that provide a robust and versatile tool to isolate or deplete mouse T-cells directly from any sample, including tissue digests such as spleen or lymph nodes, whole blood, bone marrow, and mononuclear cells (MNC).
• Rapid isolation of mouse T-cells from any sample – no columns required
• Positive isolation for molecular downstream assays or efficient depletion of mouse T cells

Excellent Yield, Purity, and Viability of Recovered Cells
Dynabeads™ Mouse pan T (Thy 1.2) are uniform, superparamagnetic beads (4.5 μm diameter) that enable easy isolation of mouse T-cells from spleen or lymph nodes, although any single-cell suspension may be used. Dynabeads™ Mouse pan T beads are coated with a primary monoclonal antibody specific for the Thy 1.2 membrane antigen expressed on peripheral T cells, thymocytes and intraepithelial T lymphocytes of all common mouse strains. Dynabeads™ Mouse pan T binds to T-cells during a short incubation period in a tube, after which the bead-bound cells are separated from unbound cells using a magnet. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the isolated T cells.

Ideal for Both Depletion and Positive Isolation for Downstream Molecular Assays
Because of the powerful magnets used for separation, Dynabeads™ Mouse pan T (Thy 1.2) can be used in viscous samples such as single cell suspensions from tissue, whole blood and bone marrow, and enable efficient depletion of mouse T-cells in about 30 minutes. Dynabeads™ Mouse pan T will also afford excellent recovery of high-purity, viable cells when used for positive isolation for downstream studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note intact cells will not be released from these beads, so if you want to isolate mouse T-cells for cell-based applications, or you need to check your samples with flow cytometry, you should use Dynabeads™ FlowComp Mouse Pan T (CD90.2) (for bead-free cells).

Select what is right for you from our range of Mouse T-Cell Isolation Products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

사양

세포 유형T Cells (Whole Population)

제품 유형Antibody Coated Bead

분리 기술Depletion, positive isolation for molecular applications

셀 수Processes ∼2 x 109 cells total

출력 생존력>95%

제품라인DYNAL™, Dynabeads™

순도 또는 품질 등급Research Grade

수량5 mL

반응성Mouse

샘플 종류Lymph Node, Blood, Spleen

배송 조건Room Temperature

시작 물질 셀 수1 x 107 cells per isolation

타겟 종Mouse

직경(미터법)4.5 μm

Unit SizeEach