
Thermo Fisher Scientific Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience
FITC 결합 Rat IgG2a kappa 단클론 동형 대조 항체로, IHC, ICC/IF, Flow Cytometry에 사용 가능. 488 nm 여기, 520 nm 방출. 친화 크로마토그래피 정제, PBS 기반 버퍼에 보관. 연구용으로만 사용.
- 카탈로그번호
- 11-4321-4x (2개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific Rat IgG2a kappa Isotype Control (eBR2a), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| Specification | Description |
|---|---|
| Host/Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBR2a |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10669560 |
Product Specific Information
The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.
Applications Reported:
This rat IgG2a isotype control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.
- Use 5 µL/test for catalog numbers ending in -41 or -42
- Use 20 µL/test for catalog numbers ending in -73
Each test size contains 0.5 µg antibody.
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL.
Cell number range: 10⁵–10⁸ cells/test (empirically determined).
Careful titration recommended for optimal performance.
Optical Properties:
- Excitation: 488 nm
- Emission: 520 nm
- Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Rat IgG2a is an immunoglobulin subclass commonly used in research and diagnostic applications.
Produced by the rat immune system, it activates complement and mediates antibody-dependent cell-mediated cytotoxicity (ADCC).
Molecular weight: ~160 kDa (two heavy chains and two light chains).
Used as a control or reference antibody in rat model experiments, flow cytometry, and immunohistochemistry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805 series
- Brilliant Violet 421–786 series
- eFluor 450–660 series
- Functional Grade
- Pacific Orange
- PE, PE-Cyanine5, 5.5, 7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436–780 series
- Custom conjugation available upon request
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