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Thermo Fisher Scientific Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience
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Thermo Fisher Scientific Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience

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FITC 결합 Rat IgG2a kappa 단클론 동형 대조 항체로, IHC, ICC/IF, Flow Cytometry에 사용 가능. 488 nm 여기, 520 nm 방출. 친화 크로마토그래피 정제, PBS 기반 버퍼에 보관. 연구용으로만 사용.

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11-4321-4x (2개 옵션)
판매단위
pk
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마지막 업데이트 2025. 08. 02. 오후 09:37
Thermo Fisher Scientific 11-4321-42 Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience 100 tests pk판매 단위 pk ·
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242,500원VAT 포함 266,750원
Thermo Fisher Scientific 11-4321-41 Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience 25 tests pk판매 단위 pk ·
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142,800원VAT 포함 157,080원

Thermo Fisher Scientific · Thermo Fisher Scientific Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience

Thermo Fisher Scientific Rat IgG2a kappa Isotype Control (eBR2a), FITC

Applications and Tested Dilution

Application Tested Dilution
Immunohistochemistry (IHC) Assay-Dependent
Immunocytochemistry (ICC/IF) Assay-Dependent
Flow Cytometry (Flow) 5 µL (0.5 µg)/test
Control (Ctrl) Assay-Dependent

Product Specifications

Specification Description
Host/Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Isotype Control
Clone eBR2a
Conjugate FITC
Excitation/Emission Max 498/517 nm
Form Liquid
Concentration 5 µL/Test
Purification Affinity chromatography
Storage buffer PBS, pH 7.2, with 0.2% BSA
Contains 0.09% sodium azide
Storage conditions 4°C, store in dark, DO NOT FREEZE!
Shipping conditions Ambient (domestic); Wet ice (international)
RRID AB_10669560

Product Specific Information

The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.

Applications Reported:
This rat IgG2a isotype control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.

  • Use 5 µL/test for catalog numbers ending in -41 or -42
  • Use 20 µL/test for catalog numbers ending in -73
    Each test size contains 0.5 µg antibody.
    A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL.
    Cell number range: 10⁵–10⁸ cells/test (empirically determined).
    Careful titration recommended for optimal performance.

Optical Properties:

  • Excitation: 488 nm
  • Emission: 520 nm
  • Laser: Blue Laser

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Rat IgG2a is an immunoglobulin subclass commonly used in research and diagnostic applications.
Produced by the rat immune system, it activates complement and mediates antibody-dependent cell-mediated cytotoxicity (ADCC).
Molecular weight: ~160 kDa (two heavy chains and two light chains).
Used as a control or reference antibody in rat model experiments, flow cytometry, and immunohistochemistry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Available Formats

  • Unconjugated
  • Alexa Fluor 488, 532, 561, 647, 660, 700
  • APC, APC-eFluor 780
  • Biotin
  • Brilliant UV 395–805 series
  • Brilliant Violet 421–786 series
  • eFluor 450–660 series
  • Functional Grade
  • Pacific Orange
  • PE, PE-Cyanine5, 5.5, 7, PE-eFluor 610
  • PerCP-Cyanine5.5, PerCP-eFluor 710
  • Super Bright 436–780 series
  • Custom conjugation available upon request

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