Thermo Fisher Scientific Phospho-FAK (Ser910) Polyclonal Antibody

Applications

Product Specifications

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. It contains a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, sites where cells attach to the extracellular matrix via integrin receptors.

Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of Grb2 and c-Src PTK with FAK, resulting in activation of ERK2 MAP kinase. In v-Src-transformed NIH3T3 cells, the association of v-Src, Grb2, and Sos with FAK is independent of adhesion to fibronectin. The Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, with Tyr925 identified as the binding site. Several transcript variants encoding different isoforms have been found for the FAK gene, with three full-length forms determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.