Thermo Fisher Scientific Ub-K6 Polyclonal Antibody
Ub-K6 결합 특이성을 가진 Rabbit Polyclonal 항체로, Western blot, ELISA, Dot blot에서 검증됨. Mouse와 Rat 시료에 반응하며, DNA 손상 및 단백질 분해 연구에 유용. 액상 형태로 -20°C에서 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1 µg/mL |
| Dot Blot (DB) | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence containing polyubiquitinated protein (K6 linkage) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.64 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914182 |
Product Specific Information
Positive test controls include: RAW264.7, C6.
Target Information
Ubiquitin exists either covalently attached to another protein or free (unanchored). When covalently bound, it is conjugated via an isopeptide bond as monoubiquitin or polyubiquitin chains linked through different Lys residues or linearly via the initiator Met.
- Lys-6-linked: DNA repair
- Lys-11-linked: ERAD and cell-cycle regulation
- Lys-29-linked: Lysosomal degradation
- Lys-33-linked: Kinase modification
- Lys-48-linked: Proteasomal degradation
- Lys-63-linked: Endocytosis, DNA-damage response, NF-κB signaling
Linear polymer chains (Met-linked) are involved in cell signaling.
Free polyubiquitin (unanchored) also plays roles in kinase activation and signaling.
[Source: Uniprot]
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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