Thermo Fisher Scientific HSF1 Monoclonal Antibody (10H8), APC
HSF1 단백질 검출용 Rat 모노클로날 항체로 APC 형광 표지 제공. Western blot, IHC, ICC, ELISA 등 다양한 응용 가능. 651/660 nm 형광 특성으로 세포 스트레스 반응 연구에 적합. 고순도 Protein G 정제, 보존제 무첨가, 4°C 보관.
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Thermo Fisher Scientific HSF1 Monoclonal Antibody (10H8), APC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
| Gel Shift (GS) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Bovine, Guinea pig, Hamster, Human, Mouse, Non-human primate, Rabbit, Rat |
| Host / Isotype | Rat / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10H8 |
| Immunogen | Purified recombinant mouse HSF1 protein, epitope mapping to amino acids 378–395 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.2, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931581 |
Additional Formats
- Unconjugated (MA5-27688)
- FITC (MA5-45128)
- PE (MA5-45130)
- PerCP (MA5-45129)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45127 was sufficient for detection of HSF1 in 20 µg of heat-shocked HeLa cell lysate by ECL immunoblot analysis using Goat anti-rat IgG: HRP as the secondary antibody.
Detects approximately 85 kDa (unstressed cell lysates) and approximately 95 kDa (heat-shocked cell lysates).
Target Information
All organisms respond to elevated temperatures and various environmental stresses by rapid synthesis of heat shock RNAs and proteins. The transcriptional activator heat shock factor (HSF) mediates heat shock gene regulation by binding to heat shock response elements (HSEs), composed of three repeats of a 5-nucleotide {nGAAn} module.
Two HSFs have been identified in human cells—HSF1 and HSF2—with 38% sequence identity. HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative reagents, while HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells.
HSF1 exists constitutively as a monomer lacking DNA-binding activity and becomes activated under stress to a nuclear-localized, trimeric state that binds DNA. Phosphorylation of HSF1 is required for maximal transcription of heat shock genes.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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