Thermo Fisher Scientific HSF1 Monoclonal Antibody (10H8), FITC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:1,000

-

Immunocytochemistry (ICC/IF)

1:200

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Gel Shift (GS)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Guinea pig, Hamster, Human, Mouse, Non-human primate, Rabbit, Rat

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

10H8

Immunogen

Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HSF1,uniProtId:P38532-1,ncbiNodeId:10090,antigenRange:378-395,antigenLength:525,antigenImageFileName:MA5-45128_HSF1_P38532-1_Rat.svg,antigenImageFileNamePDP:MA5-45128_HSF1_P38532-1_Rat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

FITC FITC FITC

View additional formats

• Unconjugated
• APC
• PE
• PerCP
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

9.1mM sodium bicarbonate/PBS, pH 7.2, with 640.91mM DMSO, 136.36mM ethanolamine

Contains

no preservative

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931582

Product Specific Information

1 µg/mL of MA5-45128 was sufficient for detection of HSF1 in 20 µg of heat shocked HeLa cell lysate by ECL immunoblot analysis using Goat anti-rat IgG: HRP as the secondary antibody|Detects approximately 85kDa (unstressed cell lysates), andapproximately 95kDa (heat shocked cell lysates).

Target Information

All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.