Thermo Fisher Scientific HSF1 Monoclonal Antibody (10H8), FITC
HSF1 단백질을 검출하기 위한 FITC 결합 단일클론 항체로, Rat IgG1 유래. Western blot, IHC, ICC/IF, ELISA, IP 등 다양한 응용에 적합. 498/517nm 형광 특성. Heat shock 관련 HSF1 연구용으로 적합.
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Thermo Fisher Scientific HSF1 Monoclonal Antibody (10H8), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
| Gel Shift (GS) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Bovine, Guinea pig, Hamster, Human, Mouse, Non-human primate, Rabbit, Rat |
| Host / Isotype | Rat / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10H8 |
| Immunogen | Purified recombinant mouse HSF1 protein (epitope mapping to amino acids 378–395) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.2, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931582 |
Additional Formats
- Unconjugated (MA5-27688)
- APC (MA5-45127)
- PE (MA5-45130)
- PerCP (MA5-45129)
- Request custom conjugation available
Product Specific Information
1 µg/mL of MA5-45128 was sufficient for detection of HSF1 in 20 µg of heat-shocked HeLa cell lysate by ECL immunoblot analysis using Goat anti-rat IgG: HRP as the secondary antibody.
Detects approximately 85 kDa (unstressed cell lysates) and approximately 95 kDa (heat-shocked cell lysates).
Target Information
All organisms respond to elevated temperatures and various environmental stresses by rapid synthesis of heat shock RNAs and proteins. The transcriptional activator heat shock factor (HSF) binds to heat shock response elements (HSEs), which consist of three repeats of a 5-nucleotide {nGAAn} module.
Two HSFs have been identified in human cells: HSF1 and HSF2. They bind to the same HSEs and share 38% sequence identity. HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative reagents, while HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells.
HSF1 exists constitutively in the cytoplasm and nucleus of unstressed cells as a monomer lacking DNA binding activity. Upon stress, HSF1 becomes activated to a nuclear-localized, trimeric state that binds DNA. Phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.
Usage Restrictions
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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