Thermo Fisher Scientific GRAMD4 (Transcription Factor) Monoclonal Antibody (PCRP-GRAMD4-1A10)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Antibody is stable for 24 months.
• Positive Control: Human liver, heart, or fetal brain.
• Cellular Localization: Mitochondrion membrane; colocalizes with COX4I1.
• Specificity Comments:
  GRAMD4 (GRAM domain-containing protein 4) maps to human chromosome 22, which houses over 500 genes. Also known as death-inducing protein (DIP), GRAMD4 is a 578 amino acid mitochondrial membrane protein involved in the p53-independent E2F-1 death pathway, often deregulated in various cancers. Overexpression triggers apoptosis via caspase-3 activation and PARP cleavage. GRAMD4 is expressed in lung tissue and lung squamous cell carcinoma (LSCC), with upregulation by E2F1 after 4-hydroxytamoxifen treatment.
  Predicted to react in Rat.

Target Information

GRAMD4, also known as Death Inducing Protein, is a 578 amino acid protein containing one GRAM domain. It is a multi-pass membrane protein localized to the mitochondrial membrane. Overexpression induces apoptosis through caspase-3 activation and PARP cleavage. GRAMD4 is expressed in lung tissue and primary LSCC, showing mitochondrial upregulation by E2F-1 after 4-hydroxytamoxifen exposure. These findings suggest GRAMD4 as a potential cancer therapy target.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.