Thermo Fisher Scientific NPC1 Monoclonal Antibody (8D10B6)
NPC1 단백질을 표적으로 하는 Thermo Fisher Scientific의 Mouse Monoclonal Antibody (Clone 8D10B6). Western blot, IHC, ELISA에 사용 가능하며, 고순도 Protein G 정제 및 PBS buffer에 0.05% sodium azide 포함. 인간 NPC1 단백질(AA 34–174) 기반 면역원으로 제작됨.
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Thermo Fisher Scientific NPC1 Monoclonal Antibody (8D10B6)
Applications and Tested Dilutions
- Western Blot (WB): 1:500–1:2,000
- Immunohistochemistry (Paraffin) (IHC-P): 1:200–1:1,000
- ELISA: 1:10,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8D10B6 |
| Immunogen | Purified recombinant fragment of human NPC1 (AA: 34–174) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787430 |
Product Specific Information
MA5-31807 has been tested in indirect ELISA.
Target Information
Mutations in the Niemann-Pick disease type C1 (NPC1) gene result in a fatal progressive neurodegenerative disorder characterized by abnormal sequestration of lipids including cholesterol and glycosphingolipids.
The NPC1 protein resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. It transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolyzed and released as free cholesterol.
NPC1, along with FTO, MC4R, and PTER, has been identified as a risk locus for early-onset and morbid adult obesity in European populations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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