Thermo Fisher Scientific TCF7 Monoclonal Antibody (2E9)
TCF7 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ELISA에 적합합니다. 인간 시료에 반응하며, PBS 용액 내 액상 형태로 제공됩니다. Affinity chromatography로 정제되었으며, -20°C에서 보관 권장됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 3 µg/mL |
| ELISA | 0.03 ng/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2E9 |
| Immunogen | TCF7 (NP_003193, aa 287–384) partial recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Protein sequence:
VIAECTLKES AAINQILGRR WHALSREEQA KYYELARKER QLHMQLYPGW SARDNYGKKK RRSREKHQES TTGGKRNAFG TYPEKAAAPA PFLPMTVL
Target Information
TCF7 (Transcriptional Factor 7)
TCF7 is a transcriptional activator involved in T-cell lymphocyte differentiation. It is essential for the survival of CD4+ and CD8+ immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot perform this role. TCF7 binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') in the promoter of the CD3E gene and may act as a feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1–4 repress transactivation mediated by TCF7 and CTNNB1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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