Thermo Fisher Scientific EN1 Recombinant Rabbit Monoclonal Antibody (ARC2225)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC2225

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 293-392 of human Engrailed 1 (Q05925) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EN1,uniProtId:Q05925-1,ncbiNodeId:9606,antigenRange:293-392,antigenLength:392,antigenImageFileName:MA5-37947_EN1_Q05925-1_Rabbit.svg,antigenImageFileNamePDP:MA5-37947_EN1_Q05925-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.51 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2897867

Product Specific Information

Positive Samples: SH-SY5Y, Mouse brain, Rat brain

Immunogen sequence: KLKKKKNEKE DKRPRTAFTA EQLQRLKAEF QANRYITEQR RQTLAQELSL NESQIKIWFQ NKRAKIKKAT GIKNGLALHL MAQGLYNHST TTVQDKDESE

Target Information

EN1 (Engrailed homolog 1) is a transcription factor expressed in embryonic ventral limb ectoderm and is essential for ventral limb patterning. En1 expression in the developing perichordal tube suggests that it may also be involved in vertebrae assembly. Loss of EN1 function in mice results in dorsal transformations of ventral paw structures and in subtle alterations along the proximal/distal limb axis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.