Thermo Fisher Scientific TGF beta-3 Polyclonal Antibody, PeproTech

Applications

• Western Blot (WB)
  Tested Dilution: 0.1–0.2 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
ALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human TGF-beta3. Anti-Human TGF-beta3-specific antibody was purified by affinity chromatography employing an immobilized Human TGF-beta3 matrix.

Western Blot:
To detect Human TGF-beta3 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human TGF-beta3 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Target Information

Transforming growth factor beta 3 (TGF beta 3) is the third member of the transforming growth factor family of cytokines, which includes TGF beta 1 and beta 2. These cytokines are secreted in precursor form consisting of a bioactive C-terminal domain attached to an N-terminal domain known as latency-associated protein (LAP). Cleavage of LAP results in the mature protein, which functions as a disulfide-linked homodimer.

TGF beta 3 is highly conserved across species, with mouse and human TGF beta 3 showing 100% sequence homology and cross-species activity. Members of this family can be expressed by most cell types and exert pleiotropic effects, including suppression of B- and T-cell effector activity, mediation of tissue healing, and inhibition of tumor proliferation. The promotion of CD4+CD25+ T-cell expansion indicates an important role in protection against autoimmunity.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.