Thermo Fisher Scientific MCL-1 Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:500–1:2,000

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for Human MCL1.

Target Information

MCL1 (Myeloid cell leukemia-1) belongs to the Bcl-2 family and is involved in programming, differentiation, and maintenance of cell viability, but not proliferation.
Isoform 1 of MCL1 inhibits apoptosis while isoform 2 promotes it. The carboxy terminal of MCL1 and Bcl-2 share significant sequence homology.

Expression of MCL1 increases upon exposure of ML-1 cells to DNA damaging agents (e.g., ionizing radiation, ultraviolet radiation, alkylating drugs) along with increases in GADD45 and Bax and a decrease in Bcl-2.
Enhanced MCL1 expression, prominently associated with mitochondria, complements continued Bcl-2 expression in ML-1 cells undergoing differentiation.

Like Bcl-2, MCL1 promotes cell viability under apoptotic conditions. MCL1 may inhibit apoptosis by heterodimerizing and neutralizing pro-apoptotic Bcl-2 family members such as Bim or Bak.
Originally identified in differentiating myeloid cells, MCL1 is expressed in multiple cell types and is essential for embryogenesis and lymphocyte development.
MCL1 exists as at least two isoforms, MCL1L and MCL1S; overexpression of MCL1S promotes cell death.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.