Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11)
마우스 CD3e 단백질을 인식하는 Armenian hamster 유래 단클론 항체로 다양한 면역분석(WB, FACS, IHC 등)에 사용 가능. 비결합형 형태로 제공되며, Protein G로 정제된 고순도 액상 제품. 단기 4°C, 장기 -20°C 보관 권장.
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Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 0.5 µg / 1×10⁶ cells | 16 publications |
| Immunoprecipitation (IP) | Assay-dependent | - |
| Neutralization (Neu) | - | 2 publications |
| Functional Assay (Functional) | Assay-dependent | - |
| Blocking Assay (BLOCK) | - | 2 publications |
| T-Cell Activation (TCA) | Assay-dependent | - |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 145-2C11 |
| Immunogen | H-2Kb sp |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539045 |
Additional Formats:
Product Specific Information
MA5-17655 targets CD3e/TCRE in FACS, FN, IHC (F), IP, TCA, and WB applications and shows reactivity with Mouse samples.
The immunogen used is h-2Kb sp.
Target Information
The CD3 subunit complex is crucial for transducing antigen-recognition signals into the cytoplasm of T cells and regulating the surface expression of the TCR complex.
T cell activation via the TCR involves CD3 gamma, delta, epsilon, and zeta subunits, which are structurally related members of the immunoglobulin superfamily encoded on chromosome 11.
CD3 subunits contain cytoplasmic tails that associate with signaling molecules via a double tyrosine-based motif.
CD3 plays roles in TCR-induced growth arrest, cell survival, and proliferation. It is expressed on 68–82% of peripheral blood lymphocytes and 65–85% of thymocytes but not on B or NK cells.
Defects in CD3 genes are associated with CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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