Thermo Fisher Scientific RAD18 Monoclonal Antibody (2G8D12)
RAD18 단백질을 인식하는 Thermo Fisher Scientific의 Mouse Monoclonal Antibody(2G8D12). Western blot, Flow cytometry, ELISA에 적합. Human 시료 반응성. Protein G 정제, 1 mg/mL 농도, PBS buffer에 0.05% sodium azide 포함.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2G8D12 |
| Immunogen | Purified recombinant fragment of human RAD18 (aa: 332–430) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898530 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
Human Rad18 (hRad18) is a homologue of S. pombe Rad18. In yeast, Rad6 and Rad18 play crucial roles in post-replication repair by filling gaps in daughter strands during replication of damaged DNA. The Rad6–Rad18 interaction is critical at early steps of lesion bypass. Rad18 forms a tight complex with Rad6 via a conserved ring-finger motif, allowing binding to damaged DNA through single-stranded DNA affinity. This complex facilitates degradation of stalled DNA replication components via the ubiquitin-conjugating activity of Rad6.
Human RAD18 maps to chromosome 3p24–25, a region frequently deleted in lung, breast, ovary, and testis cancers. The RAD18 gene encodes a 495-amino-acid protein (~56 kDa) and is ubiquitously expressed in human tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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