Thermo Fisher Scientific PYCARD Monoclonal Antibody (1C3D7)
PYCARD 단백질을 인식하는 Mouse monoclonal antibody(클론 1C3D7). Western blot, IHC, ICC/IF, Flow cytometry, ELISA에 적합. 인간 단백질에 반응하며, Protein G 정제 및 PBS buffer에 보존. 세포사멸 및 염증 반응 연구용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1C3D7 |
| Immunogen | Purified recombinant fragment of human PYCARD (aa 1–120) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term); -20°C (long term), avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898528 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
PYCARD functions as a key mediator in apoptosis and inflammation. It promotes caspase-mediated apoptosis involving caspase-8 and -9, and is involved in activation of the mitochondrial apoptotic pathway. PYCARD mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled with activation of caspase-9, -2, and -3.
It plays a central role in macrophage pyroptosis, forming the ASC pyroptosome that recruits and activates caspase-1. As an inflammasome adapter, PYCARD mediates activation of caspase-1 leading to processing and secretion of proinflammatory cytokines. It interacts with pattern recognition receptors such as NLRP2, NLRP3, AIM2, and possibly IFI16.
Additionally, it is involved in dendritic cell maturation, T-cell activation, cytoskeletal rearrangements, and transcriptional activation of cytokines and chemokines via AP-1, NF-κB, and MAPK signaling pathways. Isoforms 2 and 3 may modulate inflammasome activity differently.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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