Thermo Scientific™

LanthaScreen™ TR-FRET RAR beta Coactivator Assay Kit, goat

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15137:The LanthaScreen™ TR-FRET RAR (retinoic acid자세히 알아보기

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15137:

The LanthaScreen™ TR-FRET RAR (retinoic acid receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential RAR beta ligands as agonists of ligand-dependent coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant RAR beta ligand-binding domain (RAR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous, mix-and-read assay format.

When running the LanthaScreen™ TR-FRET RAR beta coactivator assay in agonist mode to identify agonist compounds, RAR beta-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the RAR beta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

사양

콘주게이트Tb (Terbium), FITC (Fluorescein)

검출 방법Fluorescence

용도(장비)Microplate Reader

수량800 x 20 μL assays

포장384-well Plate

어세이 입력Biochemical coregulator interaction

판독End Point

배송 조건Dry Ice

타겟 입력RAR beta, NR1B2, RARB

제품라인LanthaScreen™

용도(애플리케이션)Co-Factor Interaction Assay, TR-FRET

리간드PAR beta

제품 유형TR-FRET RAR Beta Coactivator Assay Kit

No. of Assays800 x 20 μL Assays

Unit SizeEach