Thermo Fisher Scientific RUVBL2 Monoclonal Antibody (OTI1H10), TrueMAB
Human RUVBL2 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC, ICC/IF, Flow Cytometry에 적합. 동결건조형이며, 1 mg/mL 농도. NuA4 복합체 연구 및 DNA 수선 관련 연구용으로 적합. 연구용 전용 제품.
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Thermo Fisher Scientific RUVBL2 Monoclonal Antibody (OTI1H10), TrueMAB
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1H10 |
| Immunogen | Human recombinant protein fragment (amino acids 113–370 of human RUVBL2) produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
RUVBL2 possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5'→3') activities.
It is a component of the NuA4 histone acetyltransferase complex, which regulates transcription through acetylation of histones H4 and H2A.
This complex plays roles in transcriptional activation, oncogene-mediated growth, tumor suppressor-mediated arrest, apoptosis, and DNA repair.
RUVBL2 is essential in MYC-induced oncogenic transformation and modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex, while potentially inhibiting ATF2 transcriptional activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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