Thermo Fisher Scientific RUVBL2 Monoclonal Antibody (OTI1A6), TrueMAB
RUVBL2 단백질을 인식하는 mouse monoclonal antibody로 WB, IHC, ICC/IF, Flow Cytometry에 적합. 고순도 affinity chromatography 정제 제품으로, lyophilized 형태이며 PBS buffer에 trehalose 포함. 연구용으로 DNA helicase 및 NuA4 complex 연구에 활용 가능.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A6 |
| Immunogen | Human recombinant protein fragment (aa 113–370 of human RUVBL2, produced in E. coli) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
RUVBL2 possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5′→3′) activity.
It is a component of the NuA4 histone acetyltransferase complex involved in transcriptional activation via acetylation of nucleosomal histones H4 and H2A.
This modification affects nucleosome–DNA interactions and facilitates recruitment of transcriptional regulators.
NuA4 is implicated in oncogene-mediated growth induction, tumor suppressor-mediated arrest, replicative senescence, apoptosis, and DNA repair.
RUVBL2, in association with RUVBL1 and EP400, contributes to the ATPase and helicase activities of NuA4.
It plays a role in MYC-driven oncogenic transformation and modulates LEF1/TCF1–CTNNB1 complex transcriptional activation, while potentially inhibiting ATF2 transcriptional activity.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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