Thermo Fisher Scientific 14-3-3 Pan Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 10 publications 10 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 1 publication 1 publication

ELISA (ELISA)

0.1-1.0 µg/mL

-

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat, Xenopus

Published species

Bovine, Fruit fly, Human, Mouse, Plant

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 20 amino acid synthetic peptide derived from the N-terminus of the human 14-3-3 beta/alpha protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:14-3-3 Pan,uniProtId:P63104-1,ncbiNodeId:9606,antigenRange:1,antigenLength:245,antigenImageFileName:51-0700_14-3-3_Pan_P63104-1_Rabbit.svg,antigenImageFileNamePDP:51-0700_14-3-3_Pan_P63104-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533894

Product Specific Information

This antibody is broadly reactive with members of the 14-3-3 family of proteins (~29-32 kDa). With the exception of a single conserved amino acid change, this sequence is identical in the delta and zeta isoforms. Three and four conserved amino acid differences are found in the tau and gamma isoforms, respectively.

Based on the sequence homology to the immunizing peptide, this the antibody could recognize the protein from C. elegans.

Target Information

At least seven isoforms comprise the highly conserved 14-3-3 family of homo- and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and, through interaction with Bad, prevents apoptosis. Other proteins that have been shown to bind to 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, polyoma middle-T antigen, nitrate reductase, S-raf and the IGF-1 receptor. Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be quite useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.