Thermo Fisher Scientific CD85d (ILT4) Monoclonal Antibody (42D1), NovaFluor Red 710, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.4 µg)/test

Product Specifications

Additional Formats

• APC
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Custom conjugation available

Product Specific Information

The monoclonal antibody 42D1 recognizes the CD85d (ILT4, LIR-2) protein, a member of the ILT/LIR/MIR receptor family.
CD85d is a 110 kDa single transmembrane receptor with three Ig-like domains and a cytoplasmic domain containing ITIMs (immunoreceptor tyrosine-based inhibitory motifs). Expression is limited to monocytes, macrophages, and dendritic cells. Its ligand includes non-classical class I HLA-G and -B molecules. Binding triggers interaction with SHP-1 phosphatase, contributing to immune tolerance during fetal implantation and possibly influencing organ allograft rejection.

Crosslinking of CD85d with 42D1 antibody inhibits calcium flux and enhances HLA-G tetramer staining, restoring the stimulatory activity of tolerogenic dendritic cells.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported

This antibody is reported for use in flow cytometric analysis.

Applications Tested

Pre-titrated and validated by flow cytometry using normal human peripheral blood cells.
Recommended use: 5 µL (1 µg) per test (100 µL final volume).
Cell density: 10⁵–10⁸ cells/test.

Important Notes

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability analysis.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample to antibody cocktails before combining with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

Technology

NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for optimized fluorescent signatures with minimal spillover and spread.

Excitation: 639 nm
Emission: 710 nm
Laser: 633–640 nm (Red)

Target Information

Gene: LILRB2 (ILT4)
Located at chromosomal region 19q13.4, this gene encodes a receptor belonging to the LIR family.
LILRB2 contains multiple extracellular Ig domains, a transmembrane region, and cytoplasmic ITIM motifs.
It is expressed on immune cells, binds MHC class I molecules, and transduces inhibitory signals that regulate immune activation, inflammation, and cytotoxicity.
Multiple transcript variants encoding distinct isoforms are known.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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