Thermo Fisher Scientific CD69 Monoclonal Antibody (FN50), NovaFluor Blue 610-70S, eBioscience

Thermo Fisher Scientific CD69 Monoclonal Antibody (FN50), NovaFluor Blue 610-70S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.4 µg)/test
Publications: -

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 561
• Alexa Fluor 660
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 (~30 kDa) is expressed on the cell surface as a disulfide-linked dimer and is rapidly upregulated upon activation, appearing on lymphocytes, monocytes, and platelets.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated on PHA-stimulated normal human peripheral blood cells. Recommended usage: 5 µL (0.125 µg) per test in a final volume of 100 µL with 10⁵–10⁸ cells/test.

Compatibility Note:
NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.

Blocking Buffer:
This conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), which provides enhanced performance compared to the previous CellBlox formulation. Always use CellBlox Plus Blocking Buffer in experiments involving NovaFluor conjugates.

• Add 5 µL per sample before mixing with cells.
• For single-color controls, use 5 µL per 100 µL of cell sample containing 10³–10⁸ cells.

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered nucleic acid dye structures for optimized fluorescence signatures and reduced spillover/spread.

Spectral Properties:
Excitation: 509 nm
Emission: 614 nm
Laser: 488 nm (Blue)

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It contains one or two N-linked oligosaccharides and is present on activated platelets. In normal peripheral blood, CD69 expression varies among cells and is involved in lymphocyte signal transduction. Expression is induced upon T-cell activation and may regulate proliferation. CD69 also transmits signals in NK cells and platelets. Multiple transcript variants result from alternative splicing.
Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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