Thermo Fisher Scientific CD85d (ILT4) Monoclonal Antibody (42D1), NovaFluor Red 710, eBioscience

Thermo Fisher Scientific CD85d (ILT4) Monoclonal Antibody (42D1), NovaFluor Red 710, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.4 µg)/test
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells (5 µL/test)

Product Specifications

Additional Formats

• APC
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Monoclonal antibody 42D1 recognizes CD85d (ILT4, LIR-2), a 110 kDa transmembrane receptor with 3 Ig-like domains and ITIMs.
Expression is confined to monocytes, macrophages, and dendritic cells. The ligand is non-classical class I HLA-G, -B, and G1.
Interaction with HLA-G contributes to tolerance during fetal implantation and may play a role in organ allograft rejection.

Crosslinking with 42D1 inhibits calcium flux, enhances HLA-G tetramer staining, and restores stimulating activity of tolerogenic DCs.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability testing.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining.
Add 5 µL per sample to antibody cocktails or single-color controls.

NovaFluor conjugates utilize Phiton™ technology, enabling engineered fluorescent signatures optimized for minimal spillover and spread.

Excitation: 639 nm
Emission: 710 nm
Laser: 633–640 nm (Red)

Target Information

LILRB2 (ILT4) gene encodes a receptor with inhibitory ITIM motifs that binds MHC class I molecules on antigen-presenting cells.
It transduces negative signals to suppress immune activation, helping control inflammation and prevent autoreactivity.
Multiple transcript variants exist for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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