Thermo Fisher Scientific GPR27 Polyclonal Antibody
GPR27 단백질을 표적하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Human, Mouse, Rat에 반응하며 IHC(P)에 적합. 항원 친화 크로마토그래피로 정제된 액상 형태. 단기 4°C, 장기 -20°C 보관 권장.
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Applications
Immunohistochemistry (Paraffin) [IHC (P)]
- Tested Dilution: 5 µg/mL
- Recommended antigen retrieval: Proteinase K
- Detection method: Biotinylated secondary antibody → Alkaline phosphatase-streptavidin → Chromogen
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic 19 amino acid peptide from 3rd cytoplasmic domain of human GPR27 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2551062 |
Product Specific Information
Percent identity with other species by BLAST analysis:
Human, Mouse, Rat, Elephant, Panda (100%); Opossum (89%)
For IHC(P), use proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Target Information
G protein coupled receptors (GPCRs) are a large family of receptors characterized by seven transmembrane domains. Activation by extracellular stimuli (neurotransmitters, hormones, light) induces intracellular signaling cascades mediated by heterotrimeric G proteins.
The GPR27 protein (375 amino acids) exhibits typical GPCR features including:
- Seven transmembrane domains
- Potential N-linked glycosylation sites in the N-terminal extracellular domain
- Conserved cysteine residues forming possible disulfide bridges in extracellular loops
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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