Thermo Fisher Scientific iNOS Polyclonal Antibody, Biotin
Human iNOS 단백질을 인식하는 Biotin 결합 Rabbit Polyclonal Antibody. Western blot, ELISA, Immunoprecipitation에 적합. 고순도 Affinity chromatography 정제 제품으로 안정적인 신호 검출 가능. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 1064–1114 of human inducible nitric oxide synthase (iNOS) protein |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
iNOS (Inducible Nitric Oxide Synthase) is an enzyme responsible for the production of nitric oxide (NO), a gaseous free radical that functions as a signaling molecule in vasorelaxation, neurotransmission, and cytotoxicity.
NO synthesis is mediated by the nitric oxide synthase (NOS) family, which includes three isoforms:
- nNOS (neuronal, type I)
- eNOS (endothelial, type III)
- iNOS (inducible, type II)
iNOS is expressed in various cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. It is inducible by cytokines such as IFN-γ, TNF, IL-1, IL-2, and lipopolysaccharides (LPS). After induction, iNOS shows delayed but sustained activity leading to prolonged NO production.
Dysfunction of iNOS is associated with diseases such as achalasia and impotence.
Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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