Thermo Fisher Scientific XAF1 Polyclonal Antibody
XAF1 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot 및 IHC(Paraffin) 분석에 적합하며, 액상 형태로 1 mg/mL 농도입니다. Affinity chromatography로 정제되었으며, PBS + 50% glycerol 용액에 보관됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human XAF1 (Accession Q6GPH4), corresponding to amino acid residues H223–L273. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2900766 |
Product Specific Information
Antibody detects endogenous levels of total XAF1.
Target Information
The inhibitors of apoptosis (IAP) proteins prevent cell death both in vitro and in vivo by inhibiting distinct caspases. The X-linked Inhibitor of Apoptosis (XIAP) is a key member of this family. XIAP’s caspase-inhibiting activity is negatively regulated by XIAP-associated factor 1 (XAF1) and Smac/DIABLO.
XAF1 binds to XIAP and antagonizes its caspase inhibitory activity, triggering redistribution of XIAP from the cytosol to the nucleus.
XAF1 is ubiquitously expressed in normal tissues but is present at low or undetectable levels in many different cancer cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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