Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Brilliant Ultra Violet 563, eBioscience

Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Brilliant Ultra Violet 563, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): Tested at 1.0 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 615, 661, 737, 805
• Brilliant Violet 421, 605, 650, 711
• eFluor 450, 506, 615, 660
• FITC
• Functional Grade
• NovaFluor Blue 510, 610-30S, 660-40S, 690
• NovaFluor Yellow 570
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 780
• Request custom conjugation available

Product Specific Information

The N418 monoclonal antibody reacts with mouse CD11c, the integrin alphaX. CD11c associates with beta2 integrin to form the CD11c/CD18 heterodimer, expressed by dendritic cells, intestinal intraepithelial lymphocytes (IEL), and some activated T cells. It binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes; ≤ 1.0 µg/test (100 µL final volume)
• Recommended: Empirical titration for optimal performance

Brilliant Ultra Violet™ 563 (BUV563) is a tandem dye emitting at 564 nm, suitable for UV (355 nm) laser-equipped cytometers. Ensure instrument compatibility.

Staining Panels: When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to reduce non-specific interactions.

Light Sensitivity: Protect vial and samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark with minimal brightness loss.

Excitation/Emission/Laser: 350 nm / 564 nm / Ultraviolet Laser

Trademark Notice: BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD11, with CD18, forms a heterodimer adhesion molecule.

• CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
• CD11b: Expressed in monocytes, macrophages, NK cells, some B/T cells, granulocytes
• CD11c: Expressed in monocytes, macrophages, NK cells, granulocytes, and some lymphocytes

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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