Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Brilliant Ultra Violet 563, eBioscience
Mouse CD11c 단백질을 인식하는 N418 클론 단일클론 항체로, Brilliant Ultra Violet 563 형광체가 결합되어 있어 유세포 분석에 최적화됨. 355 nm UV 레이저 기반 장비에 적합하며, 높은 특이성과 안정성을 제공. 연구용으로만 사용 가능.
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Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Brilliant Ultra Violet 563, eBioscience
Applications and Tested Dilution
- Flow Cytometry (Flow): Tested at 1.0 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Brilliant Ultra Violet™ 563 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N418 |
| Conjugate | Brilliant Ultra Violet™ 563 |
| Excitation/Emission Max | 349/563 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925364 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 615, 661, 737, 805
- Brilliant Violet 421, 605, 650, 711
- eFluor 450, 506, 615, 660
- FITC
- Functional Grade
- NovaFluor Blue 510, 610-30S, 660-40S, 690
- NovaFluor Yellow 570
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 600, 645, 780
- Request custom conjugation available
Product Specific Information
The N418 monoclonal antibody reacts with mouse CD11c, the integrin alphaX. CD11c associates with beta2 integrin to form the CD11c/CD18 heterodimer, expressed by dendritic cells, intestinal intraepithelial lymphocytes (IEL), and some activated T cells. It binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Mouse splenocytes; ≤ 1.0 µg/test (100 µL final volume)
- Recommended: Empirical titration for optimal performance
Brilliant Ultra Violet™ 563 (BUV563) is a tandem dye emitting at 564 nm, suitable for UV (355 nm) laser-equipped cytometers. Ensure instrument compatibility.
Staining Panels: When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to reduce non-specific interactions.
Light Sensitivity: Protect vial and samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark with minimal brightness loss.
Excitation/Emission/Laser: 350 nm / 564 nm / Ultraviolet Laser
Trademark Notice: BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Target Information
CD11, with CD18, forms a heterodimer adhesion molecule.
- CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
- CD11b: Expressed in monocytes, macrophages, NK cells, some B/T cells, granulocytes
- CD11c: Expressed in monocytes, macrophages, NK cells, granulocytes, and some lymphocytes
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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