
Takara TaqI (TthHB8I) restriction enzyme
TaqI (TthHB8I) 제한효소로, 65°C에서 작용하며 dam 메틸화에 의해 활성에 영향받음. 고농도 글리세롤, Mn2+, DMSO 또는 낮은 이온강도에서 star activity 발생 가능. 엄격한 품질 테스트를 거친 연구용 효소.
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TaqI (TthHB8I) Restriction Enzyme
Recognition Site
T | CG A
A GC | T
Product Information
| 항목 | 내용 |
|---|---|
| Supplied buffer | Basal |
| Effect of DNA methylation | Enzyme activity is affected by dam methylase |
| Reaction temperature | 65°C |
| Source | Escherichia coli carrying the plasmid encoding TaqI gene |
| Concentration | 4–12 units/µl |
| Substrate for unit definition | Lambda DNA |
| Star activity | Cleavage at alternative site(s) occurs in the presence of high concentrations of glycerol, Mn²⁺, DMSO, or at low ionic strength |
| Quality testing | Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting, and pKF3 cloning |
Additional Product Information
Please refer to the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications.
Kit Components List and Certificates of Analysis are available under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566
Asia Pacific: +1.650.919.7300
Europe: +33.(0)1.3904.6880
Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
© 2023 Takara Bio Inc. All Rights Reserved.
All trademarks are the property of Takara Bio Inc. or its affiliates.
Additional product and intellectual property information is available at takarabio.com
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