Thermo Fisher Scientific Phospho-eNOS (Ser632) Monoclonal Antibody (M232)
Phospho-eNOS (Ser632) 단백질 검출용 마우스 단클론 항체로, Western blot에 최적화됨. 인간, 마우스, 랫트 반응성. 비결합형 액상 형태로 안정적이며, -20°C에서 보관 가능. 연구용으로 eNOS 인산화 상태 분석에 적합.
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Thermo Fisher Scientific Phospho-eNOS (Ser632) Monoclonal Antibody (M232)
Applications and Tested Dilution
- Western Blot (WB): 1:500
Species Reactivity
- Human
- Mouse
- Rat
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M232 |
| Immunogen | Clone M232 was generated from a Phospho-eNOS (Ser-632) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-632 in mouse eNOS. This sequence is conserved in human (Ser-633) and rat (Ser-632) eNOS, and has low homology to other NOS family members. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942613 |
Product Specific Information
The antibody detects 140 and 120 kDa bands on SDS-PAGE immunoblots of human umbilical vein endothelial cells, but these bands are not observed after lambda phosphatase treatment. The 120 kDa band may represent a truncated form of eNOS.
Target Information
Nitric oxide (NO) is an inorganic, gaseous free radical that mediates various cellular messages such as vasorelaxation, neurotransmission, and cytotoxicity. NO production is catalyzed by nitric oxide synthase (NOS) enzymes, which convert L-arginine to L-citrulline and NO.
Three NOS isoforms have been identified:
- nNOS (type I): neuronal NOS
- eNOS (type III): endothelial NOS
- iNOS (type II): inducible NOS
All NOS isoforms contain calmodulin, NADPH, FAD, and FMN binding domains.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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