Thermo Fisher Scientific BIN1 Polyclonal Antibody
BIN1 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot, IHC, ICC/IF에 적합. 인간 및 마우스 반응성. 항원 친화 크로마토그래피로 정제된 액상 형태로, 안정적인 장기 보관 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 1 publication |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to amino acids 1–290 of BIN1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.34 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2552039 |
Product Specific Information
- Recommended positive controls: A431, mouse kidney
- Predicted reactivity: Mouse (92%), Rat (80%), Pig (92%), Chicken (81%), Bovine (92%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The BIN1 gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with tumor suppressor features. Isoforms expressed in the central nervous system may be involved in synaptic vesicle endocytosis and interact with dynamin, synaptojanin, endophilin, and clathrin. Isoforms expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus, activating a caspase-independent apoptotic process. Mouse studies suggest an important role in cardiac muscle development. Alternate splicing results in ten transcript variants encoding different isoforms, and aberrant splice variants have been described in tumor cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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