Thermo Fisher Scientific Pumilio 1 Polyclonal Antibody
Thermo Fisher Scientific의 Pumilio 1 Polyclonal Antibody는 인간 PUM1 단백질을 인식하는 토끼 유래 IgG 항체로, Western blot 및 Immunoprecipitation에 적합합니다. 고순도 항원 친화 크로마토그래피로 정제되었으며, 연구용으로만 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000–1:10,000
Immunoprecipitation (IP)
- Tested Dilution: 2–5 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1 and 50 of Human Pumilio 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Mouse and Orangutan
Target Information
PUM1 (pumilio homolog 1) is a sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3′-UTR of mRNA targets. It belongs to the PUF family of evolutionarily conserved RNA-binding proteins. PUM1 recognizes the RNA consensus sequence known as the Pumilio Response Element (PRE), 5′-UGUANAUA-3′, related to the Nanos Response Element (NRE).
PUM1 mediates post-transcriptional repression via recruitment of the CCR4-POP2-NOT deadenylase complex, leading to translational inhibition and mRNA degradation. Mutations in PUM1 can cause spinocerebellar ataxia 47.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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