Thermo Fisher Scientific Nanog Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.05–0.1 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

This antibody has been successfully tested in a Sandwich ELISA as both a capture and a detection antibody in combination with PA5-18406.

Target Information

NANOG (Nanog homeobox) is a divergent homeodomain protein that regulates pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is expressed in pluripotent mouse and human cell lines but absent in differentiated cells.
Human NANOG shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. It maps to gene locus 12p13.31 (human) and 6 F2 (mouse).
NANOG expression is detected in the inner cell mass of the blastocyst and is highly expressed in the undifferentiated N-Tera embryonal carcinoma cell line. It acts as a transcription regulator involved in embryonic stem cell proliferation and self-renewal.
NANOG’s role in embryonic development suggests its potential use in creating stem cells for regenerative therapies. Artificial stem cells (iPS cells) can be generated by expressing transcription factors such as NANOG, Sox2, Klf4, Lin28, and c-Myc in fibroblasts, offering virus-free methods for safer regenerative medicine applications.
Overexpression of NANOG promotes S phase entry and proliferation. Dysfunctional NANOG protein is associated with teratocarcinoma and germ cell/embryonal cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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