Thermo Fisher Scientific CD11c Monoclonal Antibody (3.9), Alexa Fluor 700, eBioscience
인간 CD11c를 인식하는 단클론 항체로, Alexa Fluor 700으로 표지되어 유세포 분석에 적합합니다. 3.9 클론은 다양한 면역세포에서 CD11c/CD18 복합체를 검출할 수 있으며, 고품질 친화 크로마토그래피 정제 및 안정한 PBS/BSA 완충액에 보관됩니다.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (1 µg)/test
- Publications: View 33 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Cynomolgus monkey, Human, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3.9 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10545455 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 737
- Brilliant UV 805
- eFluor 450
- eFluor 506
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine5.5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Product Specific Information
Description:
The 3.9 monoclonal antibody reacts with human CD11c, a 150 kDa integrin alphaX chain. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. This complex is expressed on monocytes, granulocytes, macrophages, NK cells, dendritic cells, and subsets of T and B lymphocytes. CD11c/CD18 binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion.
Applications Reported:
This antibody is suitable for flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Use at 5 µL (1 µg) per test. One test corresponds to staining a 100 µL cell sample (10⁵–10⁸ cells/test).
Fluorochrome Information:
Alexa Fluor® 700 emits at 723 nm and can be excited with a red laser (633 nm). Recommended optical setup: 685 LP mirror and 710/20 filter.
- Excitation: 633–647 nm
- Emission: 723 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD11, together with CD18, forms a heterodimer adhesion molecule.
- CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
- CD11b: Expressed by monocytes, macrophages, NK cells, some B/T cells, granulocytes
- CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and subsets of lymphocytes
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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