Thermo Fisher Scientific SPAK Polyclonal Antibody
SPAK 단백질을 인식하는 Rabbit Polyclonal 항체로, Human 시료에 반응합니다. Western blot, IHC, IP 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 4°C에서 보관하며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 425 and 450 of Human STE20/SPS1-related proline-alanine-rich protein kinase (STK39) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
STK39 (also known as STLK3) is involved in the cellular stress response pathway. It is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled co-transporters. STK39 activates the p38 MAP kinase pathway, and its interaction with p38 decreases during cellular stress. It acts as an intermediate in the response to cellular stress and is also an independent risk factor for hypertension in men, with intragenic SNPs influencing blood pressure regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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