Thermo Fisher Scientific Ki67 Monoclonal Antibody (OTI3A11), TrueMAB
인간 Ki-67 단백질을 표적으로 하는 단클론 항체로, 세포 증식 마커 분석에 적합합니다. IHC(파라핀)에서 1:150 희석 비율로 검증되었으며, 동결 건조 형태로 제공됩니다. 고순도 친화 크로마토그래피 정제, 보존제 무첨가, 연구용 전용 시약입니다.
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Thermo Fisher Scientific Ki67 Monoclonal Antibody (OTI3A11), TrueMAB
Applications
- Immunohistochemistry (Paraffin) (IHC (P)): Tested dilution 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3A11 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1160–1493 of human MKI67 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended product: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
Ki-67 is a nuclear protein expressed during multiple stages of the cell cycle—late G1, S, G2, and M phases.
It contains a forkhead-associated (FHA) domain that interacts with euchromatin at the perichromosomal layer, centromeric heterochromatin, and nucleolus.
Ki-67 exhibits cell cycle-dependent localization: perinucleolar in G1, nuclear matrix in G2, and chromosomal during M phase.
Because Ki-67 is absent in G0 cells and increases from G1 through mitosis, it serves as a reliable proliferation marker.
Ki-67 antibodies help determine the proliferative fraction in neoplasms, correlating with tumor grade and prognosis.
Commonly used as a neuronal marker of cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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