Thermo Fisher Scientific Vimentin Monoclonal Antibody (V9), Alexa Fluor 647

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Horse, Cat, Gerbil, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

V9

Immunogen

Purified vimentin from pig eye lens if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Vimentin,uniProtId:P02543-1,ncbiNodeId:9821,antigenRange:1-466,antigenLength:466,antigenImageFileName:MA5-11883-A647_Vimentin_P02543-1_House_mouse.svg,antigenImageFileNamePDP:MA5-11883-A647_Vimentin_P02543-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

View additional formats

• Unconjugated
• Alexa Fluor 420
• Alexa Fluor 488
• Alexa Fluor 555
• Alexa Fluor Plus 594
• Alexa Fluor Plus 750
• Request custom conjugation

Excitation/Emission Max

650/671 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2662891

Product Specific Information

The MA511883A647 immunogen is purified vimentin from pig eye lens.

Target Information

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.