Thermo Fisher Scientific Vimentin Monoclonal Antibody (V9), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 32 publications 32 publications

Immunohistochemistry (IHC)

-

View 106 publications 106 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Immunocytochemistry (ICC/IF)

-

View 28 publications 28 publications

Flow Cytometry (Flow)

-

View 2 publications 2 publications

Neutralization (Neu)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Horse, Cat, Gerbil, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

Published species

Bovine, Cat, Dog, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

V9

Immunogen

Purified vimentin from pig eye lens if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Vimentin,uniProtId:P02543-1,ncbiNodeId:9821,antigenRange:1-466,antigenLength:466,antigenImageFileName:MA5-11880_Vimentin_P02543-1_House_mouse.svg,antigenImageFileNamePDP:MA5-11880_Vimentin_P02543-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10979340

Product Specific Information

MA5-11880 targets Vimentin in immunohistochemistry (paraffin) applications and shows reactivity with Bovine, Canine, Chicken, Equine, Feline, Gerbil, Hamster, Human, mouse, Non-human primate, Porcine, Rabbit, and Rat samples.

The MA5-11880 immunogen is purified vimentin from pig eye lens.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH6.0, for 10-20 minutes followed by cooling at RT for 20 min.

Target Information

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.