Thermo Fisher Scientific DAXX Recombinant Rabbit Monoclonal Antibody (SC54-06)
DAXX 단백질을 인식하는 토끼 유래 재조합 단클론 항체로, Western blot, ICC/IF, Flow cytometry에 사용 가능. 높은 특이성과 일관된 생산 로트 품질을 제공하며, 인간 시료 반응성. 단백질 A 정제, 1 mg/mL 농도, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SC54-06 |
| Immunogen | Recombinant protein within Human Daxx (aa 195–313) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809522 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The specific antibody DNA sequences from immunoreactive rabbits are cloned and expressed in HEK293 cells. Individual clones are screened to select the best candidates for production.
Advantages:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to rabbit immune repertoire
Target Information
The DAXX gene encodes a multifunctional protein localized in both the nucleus and cytoplasm. It interacts with various proteins including apoptosis antigen Fas, centromere protein C, and transcription factor E26 oncogene homolog 1. In the nucleus, DAXX acts as a transcriptional repressor binding to sumoylated transcription factors. Repression can be relieved by sequestration into PML nuclear bodies or nucleoli. The protein also associates with centromeres during the G2 phase and may regulate apoptosis in the cytoplasm. Its localization and function are influenced by post-translational modifications such as sumoylation, phosphorylation, and polyubiquitination. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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