Thermo Fisher Scientific Biotin Polyclonal Antibody, FITC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:500-1:2,500

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Biotin conjugated Keyhole Limpet Hemocyanin (KLH)

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Lyophilized

Concentration

1 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Biotin Antibody Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Biotin conjugated IgG and Biotin conjugated Albumin.

Target Information

Biotin is a water-soluble B complex vitamin, generally classified as Vitamin B7. It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required for incorporation of carbon dioxide into organic compounds and exhibits a MW of ~244 Da.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.